Dept. of Biochemistry & Organic Chemistry
Enzymology and Directed Protein Evolution
Research areas
Enzymology and bioorganic catalysis
Epoxide hydrolase
- Catalytic mechanisms
- Structure/function
- Directed evolution and re-design of function
- Functional genomics of α/β-hydrolase fold enzymes
Diol dehydrogenase
- Catalytic mechanisms
- Structure/function
- Directed evolution and re-design of function
- Coupled reactions
Aldolase
- Directed evolution and re-design of function
- Coupled reactions
Protein/DNA interactions
λ Cro
- Structure/function of λ Cro
- Tailor-making binding specificity
Group members
- Mikael Widersten (Prof.)
Project leader
Directed evolution / enzymology / DNA-protein interactions - Diana Lindberg (MSc)
PhD student
Epoxide hydrolase promiscuity - Ann Gurell (MSc)
Phd student
Directed evolution and enzymology of epoxide hydrolase - Cecilia Blikstad (MSc)
PhD student
Directed evolution of diol dehydrogenase
Undergraduate/MSc projects
- Directed evolution of novel DNA-binding proteins
- Analysis and improvements of stability in non-polar solvents and at high temperatures of plant epoxide hydrolase StEH1
- Coupled enzyme-catalyzed reactions
Theses from the group
Lisa Elfström (2005)
Characterization of epoxide hydrolases from yeast and potato
Mikael Nilsson (2004)
Protein-DNA recognition. In vitro evolution and characterization of DNA-binding proteins
Alumni
- Mikael Nilsson (PhD)
Present affiliation: Dept. of Cell and Molecular Biology, UU. - Lisa Elfström (PhD)
Present affiliation: Dept. of Medical Biochemistry and Microbiology, UU