Dept. of Biochemistry & Organic Chemistry
Publications 2005-2008
Mikael Widersten: Publications
Lindberg, D., Gogoll, A. and Widersten, M. (2008) Substrate-dependent hysteretic behavior in StEH1-catalyzed hydrolysis of styrene oxide derivatives. FEBS J. 275, 6309-6320.
Thomaeus, A., Naworyta, A., Mowbray, S.L. and Widersten, M. (2008) Removal of distal protein-water hydrogen bonds in a plant epoxide hydrolase increases catalytic turnover but decreases thermostability. Protein Sci. 17, 1275-1284.
Thomaeus, A., Carlsson, J., Åqvist, J. and Widersten, M. (2007) Active site of epoxide hydrolases revisited: a non-canonical residue in potato StEH1 promotes both formation and breakdown of the alkylenzyme intermediate. Biochemistry 46, 2466-2479.
Mowbray, S.L., Elfström, L.T., Ahlgren, K.M., Andersson, C.E. and Widersten, M. (2006) X-Ray structure of potato epoxide hydrolase sheds light on substrate specificity in plant enzymes. Protein Sci. 15, 1628-1637.
Elfström, L.T. and Widersten, M. (2006) Implications for an ionized alkylenzyme intermediate during StEH1 catalyzed trans-stilbene oxide hydrolysis. Biochemistry 45, 205-212.
Jonas, K., van der Vries, E., Nilsson, M.T.I. and Widersten, M. (2005) Isolation of novel single-chain Cro proteins targeted for binding to the bcl-2 transcription initiation site by repertoire selection and subunit combinatorics. Protein Eng. Des. Sel. 18, 537-546.
Elfström, L.T and Widersten, M. (2005) Catalysis of potato epoxide hydrolase, StEH1. Biochem. J. 290, 633-640.
Elfström, L.T. and Widersten, M. (2005) The Saccharomyces cerevisiae ORF YNR064c protein has characteristics of an 'orphaned' epoxide hydrolase. Biochim. Biophys. Acta 1748, 213-221.