Dept. of Biochemistry & Organic Chemistry
Publications 1990- 1994
Mikael Widersten: Publications
Comstock, K.E., Widersten, M., Hao, X.-Y., Henner, W.D. and Mannervik, B. (1994) A comparison of the enzymatic and physicochemical properties of human glutathione transferase M4-4 with three other human Mu class enzymes. Arch. Biochem. Biophys. 311, 487-495.
Björnestedt, R., Stenberg, G., Widersten, M., Sinning, I., Jones, T.A. and Mannervik, B. Functional significance of arginine 15 in the active site of human class Alpha glutathione transferase A1-1. J. Mol. Biol. 247, 765-773.
Widersten, M., Björnestedt, R. and Mannervik, B. (1994) Contribution of residue 208 in the hydrophobic binding site to the catalytic mechanism of human glutathione transferase A1-1. Biochemistry 33, 11717-11723.
Chaga, G., Widersten, M., Andersson, L., Porath, J. and Mannervik, B. (1994) Engineering of a metal coordinating site into human glutathione transferase M1-1 based on immobilized metal ion affinity chromatography of homologous rat enzymes. Protein Eng. 7, 1115-1119.
Mannervik, B., Berhane, K., Björnestedt, R., Board, P.G., Jones, A.T., Kolm, R.H., Olin, B., Sinning, I., Sroga, G.E., Stenberg, G., Tardioli, S. and Widersten, M. (1993) Structural and functional characterization of the binding sites for glutathione (G-site) and the hydrophobic electrophilic substrate (H-site) in glutathione transferases in Structure and Function of Glutathione S-Transferases (Tew, K.D., Pickett, C.B., Mantle, T.J., Mannervik, B. and Hayes, J.D eds.) pp 29-38, CRC Press, Inc., Boca Raton, FL.
Berhane, K., Widersten, M., Engström, Å., Kozarich, J.W. and Mannervik, B. (1994) Detoxication of base propenals and other α,β-unsaturated aldehyde products of radical reactions and lipid peroxidation by human glutathione transferases. Proc. Natl. Acad. Sci. USA 91, 1480-1484.
Hao, X.-Y., Widersten, M., Ridderström, M., Hellman, U. and Mannervik, B. (1993) Covariation of glutathione transferase expression and cytostatic drug resistance in HeLa cells: Establishment of class Mu glutathione transferase M3-3 as the dominating isoenzyme. Biochem. J. 297, 59-67.
Widersten, M. (1992) Glutathione transferases-studies on the structure and function of recombinant enzymes. Acta Universitatis Upsaliensis, 383. (PhD thesis)
Kolm, R.H., Stenberg, G., Widersten, M. and Mannervik, B. (1995) High-level bacterial expression of human glutathione transferases P1-1 encoded by semi-synthetic DNA. Protein Expres. Purif. 6, 265-271.
Widersten, M., Kolm, R.H., Björnestedt, R. and Mannervik, B. (1992) Contribution of five amino acid residues in the glutathione binding site to the function of human glutathione transferase P1-1. Biochem. J. 285, 377-381.
Widersten, M. and Mannervik, B. (1992) A structural role of histidine 15 in human glutathione transferase M1-1, an amino acid residue conserved in class Mu enzymes. Protein Eng. 5, 551-557.
Mannervik, B., Awasthi, Y.C., Board, P.G., Hayes, J.D., Di Ilio, C., Ketterer, B., Listowsky, I., Morgenstern, R., Muramatsu, M., Pearson, W.R., Pickett, C.B., Sato, K., Widersten, M. and Wolf, C.R. (1992) Nomenclature for human glutathione transferases. Biochem. J. 282, 305-306.
Björnestedt, R., Widersten, M., Board, P.G. and Mannervik, B. (1992) Design of two chimaeric human-rat class Alpha glutathione transferases for probing the contribution of C- terminal segments of protein structure to the catalytic properties. Biochem. J. 282, 505-510.
Widersten, M., Holmström, E. and Mannervik, B. (1991) Cysteine residues are not essential for the catalytic activity of human class Mu glutathione transferase M1a-1a. FEBS Lett. 293, 156-159.
Widersten, M., Pearson, W.R., Engström, Å. and Mannervik, B. (1991) Heterologous expression of the allelic variant Mu-class glutathione transferases μ and ψ Biochem. J. 276, 519-524.
Widersten, M., Björnestedt, R., Holmström, E., Board, P.G., Pearson, W.R. and Mannervik, B. (1990) Structure-activity studies on mammalian glutathione transferases based on construction of point-mutated and chimeric enzymes. in Proc. Int. Symp. Microsomes and Drug Oxidations 8th, Stockholm, p 141.
Mannervik, B., Board, P.G., Berhane, K., Björnestedt, R., Castro, V.M., Danielson, U.H., Hao, X.-Y., Kolm R., Olin, B., Principato, G.B., Ridderström, M., Stenberg, G. and Widersten, M. (1990) Classes of glutathione transferases: structural and catalytic properties of the enzymes. in Glutathione S-Transferases and Drug Resistance (Hayes, J.D., Pickett, C.B. and Mantle, T.J., eds.) pp 35-46, Taylor and Francis, London.
Castro, V.M., Söderström, M., Carlberg, I., Widersten, M., Platz, A. and Mannervik, B. (1990) Differences among human tumor cell lines in the expression of glutathione-linked enzymes. Carcinogenesis 11, 1569-1576.
Mannervik, B., Widersten, M. and Board, P.G. (1990) Glutathione-linked enzymes in detoxication reactions. in Glutathione Centennial. Molecular perspectives and Clinical Implications (Tanaguchi, N., Higashi, T., Sakamoto, Y. and Meister, A., eds.) pp 23-34, Academic Press, San Diego.