Dept. of Biochemistry & Organic Chemistry
Publications 1995-1999
Mikael Widersten: Publications
Hansson, L.O., Widersten, M. and Mannervik, B. (1999) An approach to optimizing the active site in a glutathione transferase. Biochem. J. 344, 93-100.
Hansson, L.O., Bolton-Grob, R., Widersten, M. and Mannervik, B. (1999) Structural determinants in domain II of human glutathione transferase M2-2 govern the characteristic activities with aminochrome, 2-cyano-1,3-dimethyl-1-nitrosoguanidine and 1,2-dichloro-4-nitrobenzene. Protein Sci. 8, 2742-2750.
Ylinenjärvi, K., Widersten, M. and Björk, I. (1999) The wild-type N-terminal sequence is not essential for tight binding of cystatin A (stefin A) to cysteine proteases. Selection of cystatin A variants with other high-affinity N-terminal sequences by phage display. Eur. J. Biochem. 261, 682-688.
Mannervik, B., Cameron, A.D., Fernandez, E., Gustafsson, A., Hansson, L.O., Jemth, P., Jiang, F., Jones, T.A., Larsson, A.-K., Nilsson, L.O., Olin, B., Pettersson, P.L., Ridderström, M., Stenberg, G. and Widersten, M. (1998) An evolutionary approach to the design of glutathione-linked enzymes. Chem. Biol. Interact. 111-112, 15-21.
Johansson, A.-S., Stenberg, G., Widersten, M. and Mannervik, B. (1998) Structure-activity relationships and thermal stability of human glutathione transferase P1-1 governed by the H-site residue 105. J. Mol. Biol. 278, 687-798.
Sundberg, K., Johansson, A.-S., Stenberg, G., Widersten, M., Seidel, A., Mannervik, B. and Jernström, B. (1998) Differences in the catalytic activity of allelic variants of glutathione transferase P1-1 towards carcinogenic diol epoxides of polycyclic aromatic hydrocarbons. Carcinogenesis 19, 433-436.
Widersten, M. (1998) Heterologous expression in Escherichia coli of soluble active-site random mutants of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 by coexpression of molecular chaperonins GroEL/ES. Protein Expres. Purif. 13, 389-395.
Sundberg, K., Widersten, M., Seidel, A., Mannervik, B. and Jernström, B. (1997) Glutathione conjugation of bay- and fjord-region diol epoxides of polycyclic aromatic hydrocarbons by glutathione transferases M1b-1b and P1-1. Chem. Res. Toxicol. 10, 1221-1227.
Baez, S., Segura-Aguilar, J., Widersten, M., Johansson, A.-S. and Mannervik, B. (1997) Glutathione transferases catalyse the detoxication of oxidized metabolites (o-quinones) of catecholamines and may serve as an antioxidant system preventing degenerative cellular processes. Biochem. J. 324, 25-28.
Johnson, W.W., Keng, Y.-F., Widersten, M., Mannervik, B., Hayes, J.D., Sherratt, P., Ketterer, B. and Guengerich, F.P. (1997) Conjugation of highly reactive aflatoxin B1exo-8,9-epoxide catalyzed by rat and human glutathione transferases: estimation of kinetic parameters. Biochemistry 36, 3056-3060.
Segura-Aguilar, J., Baez, S., Widersten, M., Welch, C.J. and Mannervik, B. (1997) Human class Mu glutathione transferases in particular isoenzyme M2-2 catalyze detoxication of the dopamine metabolite aminochrome. J. Biol. Chem. 272, 5727-5731.
Hansson, L.O., Widersten, M. and Mannervik, B. (1997) Mechanism-based phage display selection of active-site mutants of human glutathione transferase A1-1 catalyzing SNAr reactions. Biochemistry 36, 11252-11260.
Widersten, M., Björnestedt, R. and Mannervik, B. (1996) Involvement of the carboxyl groups of glutathione to the catalytic mechanism of human glutathione transferase A1-1. Biochemistry 35, 7731-7742.
Widersten, M., Huang, M. and Mannervik, B. (1996) Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding DNA. Protein Expres. Purif. 7, 367-372.
Yilmaz, S., Widersten, M., Emahazion, T., and Mannervik, B. (1995) Generation of a Ni(II) binding site by introduction of a histidine cluster in the structure of human glutatione transferase A1-1. Protein Eng. 8, 1163-1169.
Mannervik, B., Björnestedt, R., Davey, E., Emahazion, T., Fernandez, E., Hansson, L.O., Kolm, R.H., Nilsson, L.O., Olin, B., Stenberg, G., Tardioli, S., Widersten, M., and Yilmaz, S. (1996) Glutathione transferases with novel properties generated by protein engineering and phage display. in Glutathione S-Transferases, Structure, Function and Clinical Implications (Vermeulen, N.P.E., Mulder, G.J., Nieuwenhuyse, H., Peters, W.H.M. and Van Bladeren, P.J., eds.), pp 1-11, Taylor and Francis Ltd, London, UK
Mannervik, B., Widersten, M., Björnestedt, R. and Hansson, L.O. (1995) Design of novel glutathione transferases by phage display. in Perspectives on Protein Engineering (Epton, R. and Geisow, M.J., eds.), pp 138-142, Mayflower Worldwide Ltd., Birmingham, UK.
Mannervik, B. and Widersten, M. (1995) Human glutathione transferases classification, tissue distribution, structure and functional properties. in Adv. Drug Metab. Man (Pacifici, G.M. and Fracchia, G.N., eds.) pp 408-459, Luxembourg: Office for Official Publications of the European Communities, Brussels, Belgium.
Widersten, M. and Mannervik, B. (1995) Glutathione transferases with novel active sites isolated by phage display from a library of random mutants. J. Mol. Biol. 250, 115-122.